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dark blue cathode running buffer  (Thermo Fisher)


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    Thermo Fisher dark blue cathode running buffer
    Dark Blue Cathode Running Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dark+blue+cathode+buffers/imperial+protein+stain/pm40398393-601-131-134
    Average 90 stars, based on 1 article reviews
    dark blue cathode running buffer - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Clear Native PAGE:

    Article Title: Reduction in activity and abundance of mitochondrial electron transport chain supercomplexes in pulmonary hypertension-induced right ventricular dysfunction
    Article Snippet: Equal μg of mitochondria (∼10 μg) were loaded on Invitrogen 4-16% Native-PAGE gels. .. Gels were run for 60 min at 150V with Invitrogen Native- PAGE anode and dark blue cathode buffers followed by 250V with light blue cathode buffer (Invitrogen) for 180 min according to manufacturer’s instructions. .. Gels were blotted to nitrocellulose membranes and probed for complex I (Ndufa9; 1:1000 Abcam), complex IV (Cox IV, 1:1000; Abcam) and complex III (UQCRFS1; 1:1000 Abcam), in that order with stripping in between using 10% SDS/ 2% 2-mercaptoethanol stripping buffer at 37 ° C. One-dimensional electrophoresis in SDS-PAGE was performed using whole cell lysates collected following initial dounce homogenization in MIB, protein normalization with BCA and solubilization with 4X SDS sample buffer.



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